Journal: Biotechnology Journal
Article Title: Transcriptomic Insights Into Serum‐Free Medium Adaptation and Temperature Reduction in Chinese Hamster Ovary Cell Cultures
doi: 10.1002/biot.70055
Figure Lengend Snippet: Module eigengene plots of the three datasets. The y‐axes represent the eigengene expression levels. (a) serum‐sensitive modules, (b) cell line modules, (c) temperature‐sensitive modules, (d) differences in CHO dhFr‐ expression, (e) differences in glucose samples expression. Dashed lines delineate the serum adaptation, glucose, and temperature datasets, respectively. Diagonal filled bars represents CHO dhFr‐, solid filled bars represents rCHO DP‐12, and cross‐hatched bars represents CHO K1‐PF. Number of genes within respective module provided in lower right corner. Error bars represent standard deviation of module eigengenes ( N = 2).
Article Snippet: The recombinant cell line rCHO DP‐12 clone #1934 (ATCC CRL‐12445) produces a human monoclonal antibody (IgG 1 ) against Interluekin‐8 (IL‐8); CHO dhFr‐ (ATCC CRL‐9096) is a non‐recombinant host cell line and the parental cell line of rCHO DP‐12, and; CHO K1‐PF (Sigma 00102307) is a non‐recombinant host cell line derived from CHO‐K1 and previously adapted to the Sigma CHO protein‐free medium [ , ].
Techniques: Expressing, Standard Deviation